Latent Cytomegalovirus Infection of BALB/c Mouse Spleens Detected by an Explant Culture Technique

Reactivation of latent cytomegalovirus infection in mouse brain cells detected after transfer to brain slice cultures.

Cytomegalovirus (CMV) is the most significant infectious cause of brain disorders in humans involving the developing brain. It is hypothesized that the brain disorders occur after recurrent reactivation of the latent infection in some kinds of cells in the brains. In order to test this hypothesis, we examined the reactivation of latent murine CMV (MCMV) infection in the mouse brain by transfer ...

Cytomegalovirus: a cause of persistent latent infection.

Latent cytomegalovirus infection in blood donors.

Twenty-one out of 32 apparently healthy blood donors aged 21 to 65 years yielded positive complement fixation tests with a cytomegalovirus antigen, at titres ranging from 1:8 to 1:64. Virus was present in leucocyte cultures of fresh peripheral blood of two seropositive subjects from a total of 35 donors examined. Plasma and 48-hour stored blood specimens failed to disclose virus in culture. Vir...

Latent CytomegaLovirus infeCtion among eLderLy thais

We determined the seroprevalence of latent cytomegalovirus (CMV) infection among young and elderly adults to test for a change in seroprevalence with increasing age. Thirty-two young and 32 elderly adults were tested for antiCMV IgG, T-cell subgroup analysis, mental status and daily life activity. There was no significant difference in the seroprevalence of CMV infection between the two groups ...

The dynamics of mouse cytomegalovirus-specific CD4 T cell responses during acute and latent infection.

The dynamics of mouse cytomegalovirus (MCMV)-specific CD4 T cell responses and the mechanisms by which these cells contribute to viral control are not well understood, mainly due to lack of appropriate tools to characterize MCMV-specific CD4 T cells. We therefore generated MCMV-specific CD4 T cell hybridomas, then used an MCMV expression library and overlapping peptides to identify CD4 T cell e...